genetics

Week 8

In molecular biology on Monday, Dr. Peng discussed site specific recombination of DNA. We learned the mechanism of both tyrosine and serine recombinases, as well as examples of each. On Wednesday we reviewed the two classes of transposable elements and their mechanism of mobilization (cut-and-paste vs. long terminal repeat mobilization). Finally, we went over V(D)J recombination, which is essential in the development of a functional vertebrate immune system. B cell and T cells undergo V(D)J recombination to provide immunity to a wide range of potential toxins.

Last week in biochemistry, we discussed protein metabolism, starting with an overview of the genetic code, such as codon and amino acid pairing. Next, Dr. Popescu talked about the mechanism of protein synthesis.  On Thursday, we talked about antibiotics that target translation as well as protein targeting and degradation. In bacterial physiology, we took Test 2 on Tuesday, and began a new section on Thursday on metabolism. The first part was on regulation patterns such as feedback inhibition. We discussed the trp operon as an example of positive regulation and the lac operon in E. coli as an example of negative regulation.

In genetics lab we ran a restriction length polymorphism analysis on colonies selected last week.  The results will be compared across the class to see which groups started with the same organisms.

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